Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using LATS1/2 (Phospho-Thr1079/1041) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | LATS1/2 (phospho Thr1079/1041) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WARTS, Large tumor suppressor 1, EC 2.7.11.1 |
| Product description | The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding.1) Tao W., Nat. Genet. 21:177-181(1999). |
| Immunogen | LATS1/2 (phospho Thr1079/1041) antibody was raised against a peptide sequence around phosphorylation site of threonine 1079/1041 (E-F-T (p)-F-R) derived from Human LATS1/2 . |
| Modification | p-Thr1079/1041 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LATS1, LATS2 |
|---|---|
| Alternative Names | WARTS Large tumor suppressor 1 EC 2.7.11.1 |
| Molecular Weight(MW) | 126 kDa |
| Tissue Specificity | The antibody detects endogenous levels of LATS1/2 only when phosphorylated at threonine 1079/1041. |
| Entrez Gene | 9113/26524 |
|---|---|
| Protein Accession | NP_004681.1 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using LATS1/2 (Phospho-Thr1079/1041) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚,C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
