Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Product Name | LEF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LEF1, DKFZp586H0919, TCF1ALPHA, LEF-1, TCF10, TCF7L3 |
| Product description | Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1). Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).1) Petropoulos, K., (2008) J. Exp. Med. 205 (3), 515-522. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LEF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| LEF1 antibody can be used for detection of LEF1 by ELISA at 1:312500. LEF1 antibody can be used for detection of LEF1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LEF1 |
|---|---|
| Alternative Names | LEF1 DKFZp586H0919 TCF1ALPHA LEF-1 TCF10 TCF7L3 |
| Molecular Weight(MW) | 44 kDa |
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | LEF1 antibody can be used for detection of LEF1 by ELISA at 1:312500. LEF1 antibody can be used for detection of LEF1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store LEF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
