Application
Antibody used in WB on Human Jurkat cells at 2.0 ug/ml.Application
Antibody used in IHC on Human Intestine.| Product Name | LEF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LEF1, LEF-1, TCF10, TCF7L3, TCF1ALPHA |
| Product description | Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1.1) Liu, X., et al., (2004) J. Invest. Dermatol. 123 (2), 264-274. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LEF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| LEF1 antibody can be used for detection of LEF1 by ELISA at 1:62500. LEF1 antibody can be used for detection of LEF1 by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LEF1 |
|---|---|
| Alternative Names | LEF1 LEF-1 TCF10 TCF7L3 TCF1ALPHA |
| Molecular Weight(MW) | 44 kDa |
Application
Antibody used in WB on Human Jurkat cells at 2.0 ug/ml.
Application
Antibody used in IHC on Human Intestine.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | LEF1 antibody can be used for detection of LEF1 by ELISA at 1:62500. LEF1 antibody can be used for detection of LEF1 by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store LEF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
