Application
Western blot analysis of LEMD1 in A20 cell lysate with LEMD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | LEMD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LEMD1 Antibody: CT50, LEMP-1, LEM domain-containing protein 1, LEM domain protein 1, CT50 |
| Product description | LEMD1 Antibody: LEMD1 (LEM domain-containing1) is a member of cancer-testis gene family. LEMD1 is normally expressed only in testis. Six alternatively spliced forms of LEMD1 transcripts in normal testis have been identified, but only one of the six was expressed in colorectal cancers. LEMD1 may represent a promising target antigen for immunotherapy of colorectal cancers. In addition, LEMD1 and SPATA19 are putative cancer biomarkers and promising targets for active immunotherapy.1) Yuki D, Lin YM, Fujii Y, et al. Isolation of LEM domain-containing 1, a novel testis-specific gene expressed in colorectal cancers. Oncol. Rep. 2004; 12:275-80 |
| Immunogen | LEMD1 antibody was raised against a 19 amino acid synthetic peptide near the center of human LEMD1. The immunogen is located within amino acids 90 - 140 of LEMD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| LEMD1 antibody can be used for detection of LEMD1 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LEMD1 |
|---|---|
| Alternative Names | LEMD1 Antibody: CT50 LEMP-1 LEM domain-containing protein 1 LEM domain protein 1 CT50 |
| Tissue Specificity | At least six isoforms of LEMD1 are known to exist; this antibody will detect the longest isoform. LEMD1 antibody is predicted to not cross-react with LEMD2 and LEMD3. |
Application
Western blot analysis of LEMD1 in A20 cell lysate with LEMD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | LEMD1 antibody can be used for detection of LEMD1 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LEMD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LEMD1 Antibody is supplied in PBS containing 0.02% sodium azide. |
