Application
Immunocytochemistry of LEMD2 in 3T3 cells with LEMD2 antibody at 2.5 μg/mL.Application
Immunofluorescence of LEMD2 in 3T3 cells with LEMD2 antibody at 20 μg/mL.Application
Western blot analysis of LEMD2 in 293 cell lysate with LEMD2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | LEMD2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LEMD2 Antibody: NET25, dJ482C21.1, LEM domain-containing protein 2, hLEM2 |
| Product description | LEMD2 Antibody: The LEM (lamina-associated polypeptide-emerin-MAN1) domain is a motif shared by a group of lamin-interacting proteins of the inner nuclear membrane (INM) and nucleoplasm. One such protein, LEMD2 (LEM domain-containing protein 2), is a lamina-associated protein in the INM involved in nuclear structure organization, containing an N-terminal LEM motif, two transmembrane domains, and a MAN1-Src1p C-terminal (MSC) domain. LEMD2 is a truncated paralog of MAN1, lacking the MAN1-specific C-terminal RNA-recognition motif, but like MAN1 is required for myogenic differentiation. Mutations in LEMD2 are thought be among the possible causes of muscle disease.1) Brachner A, Reipert S, Foisner R, et al. LEM2 is a novel MAN1-related inner nuclear membrane protein associated with A-type lamins. J. Cell Sci. 2005; 118:5797-810 |
| Immunogen | LEMD2 antibody was raised against a 16 amino acid synthetic peptide near the center of human LEMD2. The immunogen is located within amino acids 220 - 270 of LEMD2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| LEMD2 antibody can be used for detection of LEMD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LEMD2 |
|---|---|
| Alternative Names | LEMD2 Antibody: NET25 dJ482C21.1 LEM domain-containing protein 2 hLEM2 |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Tissue Specificity | At least two isoforms of LEMD2 are known to exist; this antibody will detect only the longer isoform. |
Application
Immunocytochemistry of LEMD2 in 3T3 cells with LEMD2 antibody at 2.5 μg/mL.
Application
Immunofluorescence of LEMD2 in 3T3 cells with LEMD2 antibody at 20 μg/mL.
Application
Western blot analysis of LEMD2 in 293 cell lysate with LEMD2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | LEMD2 antibody can be used for detection of LEMD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LEMD2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LEMD2 Antibody is supplied in PBS containing 0.02% sodium azide. |
