Application
Immunohistochemistry of LEMD3 in human colon tissue with LEMD3 antibody at 2.5 μg/mL.Application
Western blot analysis of LEMD3 in human colon tissue lysate with LEMD3 antibody at 1 μg/mL.Application
Immunofluorescence of LEMD3 in human colon tissue with LEMD3 antibody at 5 μg/mL.| Product Name | LEMD3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LEMD3 Antibody: MAN1, MAN1, Inner nuclear membrane protein Man1, LEM domain-containing protein 3 |
| Product description | LEMD3 Antibody: LEMD3 (LEM domain-containing 3) is a lem-domain containing protein, also known as MAN1, whose loss of function results in disorders characterized by osteopoikilosis, Buschke-Ollendorff syndrome, and melorheostosis. LEMD3 is an inner nuclear membrane protein that interacts with BMP and activin-TGF-beta receptor-activated Smads and antagonizes TGF-beta signaling in human cells.1) Hellemans J, Preobrazhenska O, Willaert A, et al. Loss-of-function mutations in LEMD3 result in osteopoikilosis, Buschke-Ollendorff syndrome, and melorheostosis. Nat. Genet. 2004; 36:1213-8. |
| Immunogen | LEMD3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human LEMD3. The immunogen is located within the last 50 amino acids of LEMD3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LEMD3 antibody can be used for detection of LEMD3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LEMD3 |
|---|---|
| Alternative Names | LEMD3 Antibody: MAN1 MAN1 Inner nuclear membrane protein Man1 LEM domain-containing protein 3 |
| Tissue Specificity | At least three isoforms of LEMD3 are known to exist; this antibody will detect all isoforms. LEMD3 antibody is predicted to not cross-react with LEMD1 and LEMD2 |
Application
Immunohistochemistry of LEMD3 in human colon tissue with LEMD3 antibody at 2.5 μg/mL.
Application
Western blot analysis of LEMD3 in human colon tissue lysate with LEMD3 antibody at 1 μg/mL.
Application
Immunofluorescence of LEMD3 in human colon tissue with LEMD3 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1320 - Human Colon Tissue Lysate |
|---|---|
| Application Notes | LEMD3 antibody can be used for detection of LEMD3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LEMD3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LEMD3 Antibody is supplied in PBS containing 0.02% sodium azide. |
