Application
Western blot analysis of Leo1 using rabbit polyclonal Leo1 Antibody (Human N-term) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Leo1 gene (Lane 2).Application
Western Blot at 1:1000-1:2000 dilution Lane 1: human skeletal muscle lysate Lane 2: mouse heart lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of anti-Leo1 Antibody in HL60 cell line lysates (35ug/lane)| Product Name | LEO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. It may be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts.1) Zhao,L., FASEB J. 19 (6), 521-532 (2005) |
| Immunogen | This LEO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-159 amino acids from the N-terminal region of human LEO1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000-1:2000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LEO1 |
|---|---|
| Alternative Names | RNA polymerase-associated protein LEO1 Replicative senescence down-regulated leo1-like protein LEO1 RDL |
| Molecular Weight(MW) | 75 kDa |
Application
Western blot analysis of Leo1 using rabbit polyclonal Leo1 Antibody (Human N-term) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Leo1 gene (Lane 2).
Application
Western Blot at 1:1000-1:2000 dilution Lane 1: human skeletal muscle lysate Lane 2: mouse heart lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of anti-Leo1 Antibody in HL60 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
