Application
LGALS3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).Application
Western Blot at 1:1000 dilution + U118MG whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:1000 dilution + U118MG whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | LGALS3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, LGALS3, MAC2 |
| Product description | This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.1) Salomonsson, E., et al. J. Biol. Chem. 285(45):35079-35091(2010) |
| Immunogen | This LGALS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 167-196 amino acids from the C-terminal region of human LGALS3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LGALS3 |
|---|---|
| Alternative Names | Galectin-3 Gal-3 35 kDa lectin Carbohydrate-binding protein 35 CBP 35 Galactose-specific lectin 3 Galactoside-binding protein GALBP IgE-binding protein L-31 Laminin-binding protein Lectin L-29 Mac-2 antigen LGALS3 MAC2 |
| Molecular Weight(MW) | 26 kDa |
Application
LGALS3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution + U118MG whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:1000 dilution + U118MG whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
