Application
Immunofluorescence of LGI1 in Human Brain cells with LGI1 antibody at 20 μg/mL.Application
Immunohistochemical staining of human brain tissue using LGI1 antibody at 2.5 μg/mL.Application
Western blot analysis of LGI1 in HeLa cell lysate with LGI1 antibody at 2 μg/mL.| Product Name | LGI1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LGI1 Antibody: EPT, ETL1, ADLTE, ADPAEF, ADPEAF, IB1099, EPITEMPIN, EPT, UNQ775/PRO1569, Leucine-rich glioma-inactivated protein 1, Epitempin-1 |
| Product description | LGI1 Antibody: The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist; this LGI1 antibody will recognize only the longer form.1) Chernova OB, Somerville RP and Cowell JK. A novel gene, LGI1, from 10q24 is rearranged and downregulated in malignant brain tumors. Oncogene1998; 17:2873-81. |
| Immunogen | LGI1 antibody was raised against a 14 amino acid synthetic peptide from near the center of human LGI1. The immunogen is located within amino acids 370 - 420 of LGI1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LGI1 antibody can be used for the detection of LGI1 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LGI1 |
|---|---|
| Alternative Names | LGI1 Antibody: EPT ETL1 ADLTE ADPAEF ADPEAF IB1099 EPITEMPIN EPT UNQ775/PRO1569 Leucine-rich glioma-inactivated protein 1 Epitempin-1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | This LGI1 antibody is predicted to be specific to LGI1 and not recognize other LGI proteins. |
Application
Immunofluorescence of LGI1 in Human Brain cells with LGI1 antibody at 20 μg/mL.
Application
Immunohistochemical staining of human brain tissue using LGI1 antibody at 2.5 μg/mL.
Application
Western blot analysis of LGI1 in HeLa cell lysate with LGI1 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | LGI1 antibody can be used for the detection of LGI1 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LGI1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LGI1 Antibody is supplied in PBS containing 0.02% sodium azide. |
