Application
Immunohistochemical staining of human brain tissue using LGI2 antibody at 2.5 μg/mL.Application
Immunofluorescence of LGI2 in Human Brain cells with LGI2 antibody at 5 μg/mL.Application
Western blot analysis of LGI2 in mouse liver tissue lysate with LGI2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | LGI2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LGI2 Antibody: mKIAA1916, Kiaa1916, Leucine-rich repeat LGI family member 2, Leucine-rich glioma-inactivated protein 2 |
| Product description | LGI2 Antibody: The leucine-rich, glioma inactivated gene 2 (LGI2) is a member of the LGI family in which LGI1 is the exemplar. The LGI family consists of four of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 has been identified as a candidate tumor suppressor gene for glioma and plays a role in autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Despite its high homology with LGI1 and similar pattern of expression, mutations in LGI2 have not been found to be associated with ADTLE.1) Gu W, Gibert Y, Wirth T, et al. Using gene-history and expression analysis to assess the involvement of LGI genes in human disorders. Mol. Biol. Evol.2005; 22:2209-16. |
| Immunogen | LGI2 antibody was raised against a 10 amino acid synthetic peptide from near the center of human LGI2. The immunogen is located within amino acids 290 - 340 of LGI2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LGI2 antibody can be used for the detection of LGI2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Lgi2 |
|---|---|
| Alternative Names | LGI2 Antibody: mKIAA1916 Kiaa1916 Leucine-rich repeat LGI family member 2 Leucine-rich glioma-inactivated protein 2 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | This LGI2 antibody is predicted to be specific to LGI2 and not recognize other LGI proteins.Three isoforms of LGI2 are known to exist; this LGI2 antibody will recognize only the longest. |
Application
Immunohistochemical staining of human brain tissue using LGI2 antibody at 2.5 μg/mL.
Application
Immunofluorescence of LGI2 in Human Brain cells with LGI2 antibody at 5 μg/mL.
Application
Western blot analysis of LGI2 in mouse liver tissue lysate with LGI2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1404 - Mouse Liver Tissue Lysate |
|---|---|
| Application Notes | LGI2 antibody can be used for the detection of LGI2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LGI2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LGI2 Antibody is supplied in PBS containing 0.02% sodium azide. |
