Application
Western blot analysis of LGI4 in human brain tissue lysate with LGI4 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | LGI4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LGI4 Antibody: LGIL3, LGIL3, UNQ6515/PRO21485, Leucine-rich repeat LGI family member 4, LGI1-like protein 3 |
| Product description | LGI4 Antibody: The leucine-rich, glioma inactivated gene 4 (LGI4) is a member of the LGI family in which LGI1 is the exemplar. The LGI family consists of four of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 has been identified as a candidate tumor suppressor gene for glioma and plays a role in autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Despite its high homology with LGI1 and similar pattern of expression, mutations in LGI4 have not been found to be associated with ADTLE. However, the LGI4 gene is located in a region linked to benign familial infantile convulsions. Further study revealed that a GC-to-AT polymorphism was correlated with childhood absence epilepsy. Other studies showed that decreasing LGI4 expression in cultured cells inhibits myelination, indicating that LGI4 may play a role in neural development.1) Gu W, Gibert Y, Wirth T, et al. Using gene-history and expression analysis to assess the involvement of LGI genes in human disorders. Mol. Biol. Evol.2005; 22:2209-16. |
| Immunogen | LGI4 antibody was raised against a 14 amino acid synthetic peptide from near the center of human LGI4. The immunogen is located within amino acids 240 - 290 of LGI4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| LGI4 antibody can be used for the detection of LGI4 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LGI4 |
|---|---|
| Alternative Names | LGI4 Antibody: LGIL3 LGIL3 UNQ6515/PRO21485 Leucine-rich repeat LGI family member 4 LGI1-like protein 3 |
| Tissue Specificity | Two isoforms of LGI4 are known to exist; this LGI4 antibody will recognize only the larger form. This LGI4 antibody is predicted to be specific to LGI4 and not recognize other LGI proteins. |
Application
Western blot analysis of LGI4 in human brain tissue lysate with LGI4 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | LGI4 antibody can be used for the detection of LGI4 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LGI4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LGI4 Antibody is supplied in PBS containing 0.02% sodium azide. |
