Application
Western blot analysis of LIF in 3T3 cell lysate with LIF antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of LIF in 3T3 cells with LIF antibody at 20 μg/mL.| Product Name | LIF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIF Antibody: CDF, DIA, HILDA, MLPLI, Leukemia inhibitory factor, Differentiation-stimulating factor, LIF |
| Product description | LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.1) Trouillas M, Saucourt C, Guillotin B, et al. The LIF cytokine: towards adulthood. Eur. Cytokine Netw. 2009; 20:51-62. |
| Immunogen | LIF antibody was raised against a 16 amino acid synthetic peptide near the center of human LIF. The immunogen is located within amino acids 50 - 100 of LIF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| LIF antibody can be used for detection of LIF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LIF |
|---|---|
| Alternative Names | LIF Antibody: CDF DIA HILDA MLPLI Leukemia inhibitory factor Differentiation-stimulating factor LIF |
| Molecular Weight(MW) | Predicted: 22 kDa Observed: 25 kDa |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Tissue Specificity | At least two isoforms of LIF are known to exist. |
Application
Western blot analysis of LIF in 3T3 cell lysate with LIF antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of LIF in 3T3 cells with LIF antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | LIF antibody can be used for detection of LIF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LIF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LIF Antibody is supplied in PBS containing 0.02% sodium azide. |
