Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Product Name | LIG3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA ligase 3, DNA ligase III, Polydeoxyribonucleotide synthase [ATP] 3, LIG3 |
| Product description | This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.1) Wang, W., et al. Nucleic Acids Res. (2010) In press : |
| Immunogen | This LIG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 793-822 amino acids from the C-terminal region of human LIG3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LIG3 |
|---|---|
| Alternative Names | DNA ligase 3 DNA ligase III Polydeoxyribonucleotide synthase [ATP] 3 LIG3 |
| Molecular Weight(MW) | 113 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
