Application
WB ImageWhole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [6G9] diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.Application
WB ImageSample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 7.5% SDS PAGE diluted at 1:1000| Product Name | DNA ligase III antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [6G9] to DNA ligase IIIThere are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97kDa (§-form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in the base excision repair pathway. |
| Immunogen | Recombinant full-length human DNA Ligase III-b. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6G9 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| For IHC-P: Use at a dilution of 1:10 for 30 minutes. For WB: Use at a concentration of 1-5 µg/ml. Predicted molecular weight: 103 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LIG3 P49916 DNA ligase III 3980 600940 |
|---|---|
| Target | DNA ligase III |
| Molecular Weight(MW) | 100 kDa (note) |
| Cellular Localization | Mitochondrial and Nuclear |
| Light Chain Type | kappa |
Application
WB ImageWhole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [6G9] diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.
Application
WB ImageSample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 7.5% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , PC-3 , U87-MG , SK-N-SH |
|---|---|
| Application Notes | For IHC-P: Use at a dilution of 1:10 for 30 minutes. For WB: Use at a concentration of 1-5 µg/ml. Predicted molecular weight: 103 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing no preservativs |
