Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.Application
Antibody used in IHC on Human Pancreas.| Product Name | LIG4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIG4, |
| Product description | LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V (D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.1) Smith, J., et al., (2003) Nucleic Acids Res. 31 (8), 2157-2167. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LIG4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| LIG4 antibody can be used for detection of LIG4 by ELISA at 1:312500. LIG4 antibody can be used for detection of LIG4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LIG4 |
|---|---|
| Alternative Names | LIG4 |
| Molecular Weight(MW) | 104 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Application
Antibody used in IHC on Human Pancreas.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | LIG4 antibody can be used for detection of LIG4 by ELISA at 1:312500. LIG4 antibody can be used for detection of LIG4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store LIG4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
