EPLIN is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. EPLIN is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles (Maul et al., 2003 [PubMed 12566430]).1) Abe, K. (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (1), 13-19.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LIMA1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
LIMA1 antibody can be used for detection of LIMA1 by ELISA at 1:62500. LIMA1 antibody can be used for detection of LIMA1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Placenta at 0.2-1 ug/ml.
Positive Control
1) 293T Cell Lysate
Application Notes
LIMA1 antibody can be used for detection of LIMA1 by ELISA at 1:62500. LIMA1 antibody can be used for detection of LIMA1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store LIMA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.