Application
Immunohistochemistry of LIMA1 in human colon tissue with LIMA1 antibody at 5 μg/mL.Application
Immunofluorescence of LIMA1 in human colon tissue with LIMA1 antibody at 20 μg/mL.Application
Western blot analysis of LIMA1 in human colon tissue lysate with LIMA1 antibody at (A) 0.5 and (B) 1 μg/ml.| Product Name | LIMA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIM domain and actin-binding protein 1, PP624, EPLIN, SREBP3 |
| Product description | The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).1) Maul RS and Chang DD. EPLIN, epithelial protein lost in neoplasm. Oncogene 1999; 18:7838-41. |
| Immunogen | LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1. The immunogen is located within amino acids 230 - 280 of LIMA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LIMA1 |
|---|---|
| Alternative Names | LIM domain and actin-binding protein 1 PP624 EPLIN SREBP3 |
| Molecular Weight(MW) | Predicted: 50, 66, 84 kDa Observed: 65 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (89%) |
| Tissue Specificity | LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist; this antibody will only detect the three largest isoforms. |
| Entrez Gene | 51474 |
|---|---|
| Protein Accession | NP_001107018 |
Application
Immunohistochemistry of LIMA1 in human colon tissue with LIMA1 antibody at 5 μg/mL.
Application
Immunofluorescence of LIMA1 in human colon tissue with LIMA1 antibody at 20 μg/mL.
Application
Western blot analysis of LIMA1 in human colon tissue lysate with LIMA1 antibody at (A) 0.5 and (B) 1 μg/ml.| Positive Control | 1) Cat. No. 1320 - Human Colon Tissue Lysate |
|---|---|
| Application Notes | LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LIMA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | LIMA1 antibody is supplied in PBS containing 0.02% sodium azide. |
