Application
Immunohistochemistry of LIMP2 in human skeletal muscle tissue with LIMP2 antibody at 10 μg/mL.Application
Western blot analysis of LIMP2 in human skeletal muscle tissue lysate with LIMP2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | LIMP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIMP2 Antibody: AMRF, EPM4, LGP85, CD36L2, HLGP85, LIMP-2, LIMPII, SR-BII, LIMP2, Lysosome membrane protein 2, 85 kDa lysosomal membrane sialoglycoprotein |
| Product description | LIMP2 Antibody: The lysosomal integral membrane protein 2 (LIMP2) is a heavily glycosylated type III transmembrane protein, the majority of which exists in the lumen of the lysosome and a cytoplasmic domain of approximately 20 amino acids. A deficiency of LIMP2 in mice causes uretic pelvic junction obstruction, deafness, and peripheral neuropathy associated with impaired vesicular trafficking and distribution of apically expressed proteins. More recently, LIMP2 was shown to act as a receptor to bind beta-glucocerebrosidase, the enzyme defective in Gaucher disease, a lysosomal storage disorder. LIMP2-deficient mice showed missorted as well as secreted beta-glucocerebrosidase, suggesting that LIMP2 also functions as the mannose-6-phosphate-independent trafficking receptor.1) Fujita H, Saeki M, Yasunaga K, et al. Isolation and sequencing of a cDNA clone encoding 85kDa sialoglycoprotein in rat liver lysosomal membranes. Biochem. Biophys. Res. Commun.1991; 178:444-52. |
| Immunogen | LIMP2 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human LIMP2. The immunogen is located within the last 50 amino acids of LIMP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| LIMP2 antibody can be used for detection of LIMP2 by Western blot at 1 and 2 μg/mL. Despite its predicted molecular weight, LIMP2 runs at approximately 80 - 85 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SCARB2 |
|---|---|
| Alternative Names | LIMP2 Antibody: AMRF EPM4 LGP85 CD36L2 HLGP85 LIMP-2 LIMPII SR-BII LIMP2 Lysosome membrane protein 2 85 kDa lysosomal membrane sialoglycoprotein |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (89%) |
Application
Immunohistochemistry of LIMP2 in human skeletal muscle tissue with LIMP2 antibody at 10 μg/mL.
Application
Western blot analysis of LIMP2 in human skeletal muscle tissue lysate with LIMP2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate |
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| Application Notes | LIMP2 antibody can be used for detection of LIMP2 by Western blot at 1 and 2 μg/mL. Despite its predicted molecular weight, LIMP2 runs at approximately 80 - 85 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LIMP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LIMP2 Antibody is supplied in PBS containing 0.02% sodium azide. |
