Application
Immunohistochemistry of LIMP2 in human skeletal muscle tissue with LIMP2 antibody at 2.5 μg/mL.Application
Immunofluorescence of LIMP2 in Human Skeletal Muscle tissue with LIMP2 antibody at 20 μg/mL.Application
Western blot analysis of LIMP2 in mouse liver tissue lysate with LIMP2 antibody at 1 μg/mL in (A) the absence and (B) presence of blocking peptide.| Product Name | LIMP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | LIMP2 Antibody: The lysosomal integral membrane protein 2 (LIMP2) is a heavily glycosylated type III transmembrane protein, the majority of which exists in the lumen of the lysosome and a cytoplasmic domain of approximately 20 amino acids. A deficiency of LIMP2 in mice causes uretic pelvic junction obstruction, deafness, and peripheral neuropathy associated with impaired vesicular trafficking and distribution of apically expressed proteins. More recently, LIMP2 was shown to act as a receptor to bind beta-glucocerebrosidase, the enzyme defective in Gaucher disease, a lysosomal storage disorder. LIMP2-deficient mice showed missorted as well as secreted beta-glucocerebrosidase, suggesting that LIMP2 also functions as the mannose-6-phosphate-independent trafficking receptor.1) Fujita H, Saeki M, Yasunaga K, et al. Isolation and sequencing of a cDNA clone encoding 85kDa sialoglycoprotein in rat liver lysosomal membranes. Biochem. Biophys. Res. Commun. 1991; 178:444-52. |
| Immunogen | LIMP2 antibody was raised against a 16 amino acid synthetic peptide from near the center of human LIMP2. The immunogen is located within amino acids 70 - 120 of LIMP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LIMP2 antibody can be used for detection of LIMP2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SCARB2 |
|---|---|
| Alternative Names | LIMP2 Antibody: AMRF EPM4 LGP85 CD36L2 HLGP85 LIMP-2 LIMPII SR-BII LIMP2 Lysosome membrane protein 2 85 kDa lysosomal membrane sialoglycoprotein |
| Molecular Weight(MW) | Predicted: 53 kDa Observed: 57 kDa |
Application
Immunohistochemistry of LIMP2 in human skeletal muscle tissue with LIMP2 antibody at 2.5 μg/mL.
Application
Immunofluorescence of LIMP2 in Human Skeletal Muscle tissue with LIMP2 antibody at 20 μg/mL.
Application
Western blot analysis of LIMP2 in mouse liver tissue lysate with LIMP2 antibody at 1 μg/mL in (A) the absence and (B) presence of blocking peptide.| Positive Control | 1) Cat. No. 1404 - Mouse Liver Tissue Lysate |
|---|---|
| Application Notes | LIMP2 antibody can be used for detection of LIMP2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | LIMP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LIMP2 Antibody is supplied in PBS containing 0.02% sodium azide. |
