Application
Immunocytochemistry of LIN54 in HeLa cells with LIN54 at 2.5 μg/mL.Application
Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 μg/ml.Application
Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 μg/ml.Application
Immunocytochemistry of LIN54 in HeLa cells with LIN54 antibody at 10 μg/ml.Application
Western blot analysis of LIN54 in HeLa cell lysate with LIN54 antibody at 1 μg/ml.| Product Name | LIN54 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIN-54, JC8.6, CXCDC1, MIP120 |
| Product description | LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes. This complex functions as a transcriptional repressor by binding to promoters of E2F target genes that regulate the G1/S transition (1). LIN54 is required for cell cycle progression and can bind to the cdc2 promoter in a sequence-specific manner (2).1) Schmit F, Cremer S, and Gaubatz S. LIN54 is an essential core subunit of the DREAM/LINC complex that binds to the cdc2 promotoer in a sequence-specific manner. FEBS J. 2009; 276:5703-16. |
| Immunogen | LIN54 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LIN54. The immunogen is located within the last 50 amino acids of LIN54. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LIN54 |
|---|---|
| Alternative Names | LIN-54 JC8.6 CXCDC1 MIP120 |
| Molecular Weight(MW) | Predicted: 88 kDa Observed: 82 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | LIN54 antibody is human, mouse, and rat reactive. Multiple isoforms of LIN54 are known to exist. |
Application
Immunocytochemistry of LIN54 in HeLa cells with LIN54 at 2.5 μg/mL.
Application
Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 μg/ml.
Application
Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 μg/ml.
Application
Immunocytochemistry of LIN54 in HeLa cells with LIN54 antibody at 10 μg/ml.
Application
Western blot analysis of LIN54 in HeLa cell lysate with LIN54 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL: |
| Form | Liquid |
|---|---|
| Storage Instructions | LIN54 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | LIN54 antibody is supplied in PBS containing 0.02% sodium azide. |
