Application
Immunocytochemistry of LIS1 in Jurkat cells with LIS1 antibody at 2.5 μg/mL.Application
Western blot analysis of LIS1 in HeLa cell lysate with LIS1 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of LIS1 in Jurkat cells with LIS1 antibody at 20 μg/mL.| Product Name | LIS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIS1 Antibody: MDS, LIS1, LIS2, MDCR, PAFAH, MDS, PAFAHA, Platelet-activating factor acetylhydrolase IB subunit alpha, Lissencephaly-1 protein, LIS-1 |
| Product description | LIS1 Antibody: Lissencephaly is a severe brain developmental disease characterized by the mislocalization of cortical neurons, a smooth cerebral surface, mental retardation, and seizures. Classical lissencephaly is caused by sporadic mutations in the LIS1 gene. While LIS1 is known to act in a pathway deactivating the lipid messenger platelet-activating factor, LIS1 forms a complex with Nudel and 14-3-3epsilon which is then transported from neuronal cell bodies through the actions of DISC1 and KIF5A, a microtubule-dependent directed motor protein kinesin. Decreased expression of LIS1 blocked neural stem cell division, morphogenesis, and motility, suggesting that LIS1 plays an important role in neuronal cell proliferation and localization in the developing brain. At least two isoforms of LIS1 are known to exist.1) McManus MF and Golden JA. Neuronal migration in developmental disorders. J. Child Neurol.2005; 20:280-6. |
| Immunogen | LIS1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human LIS1. The immunogen is located within amino acids 340 - 390 of LIS1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| LIS1 antibody can be used for detection of LIS1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAFAH1B1 |
|---|---|
| Alternative Names | LIS1 Antibody: MDS LIS1 LIS2 MDCR PAFAH MDS PAFAHA Platelet-activating factor acetylhydrolase IB subunit alpha Lissencephaly-1 protein LIS-1 |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
Application
Immunocytochemistry of LIS1 in Jurkat cells with LIS1 antibody at 2.5 μg/mL.
Application
Western blot analysis of LIS1 in HeLa cell lysate with LIS1 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of LIS1 in Jurkat cells with LIS1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | LIS1 antibody can be used for detection of LIS1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LIS1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LIS1 Antibody is supplied in PBS containing 0.02% sodium azide. |
