Application
Immunofluorescence of Livin in Human Small Intestine cells with Livin antibody at 20 μg/mL.Application
Immunohistochemistry of Livin in human small intestine tissue with Livin antibody at 5 μg/mL.Application
Western blot analysis of Livin expression in human Raji cell lysate with Livin antibody at 0.5 μg/mL.| Product Name | Livin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Livin Antibody: KIAP, LIVIN, MLIAP, RNF50, ML-IAP, KIAP, UNQ5800/PRO19607/PRO21344, Baculoviral IAP repeat-containing protein 7, Kidney inhibitor of apoptosis protein |
| Product description | Livin Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel member in the IAP protein family was recently identified and designated Livin and KIAP for kidney IAP. Livin/XIAP contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin-alpha and Livin-beta. Transfection of Livin in cells resulted in protection from apoptosis induced by FADD, BAX, RIP, RIP3 and DR6. Livin has direct interaction with several caspases including caspase-3, -7, and -9. Livin inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. The two isoforms of Livin appear to have different functions and tissue distributions.1) Kasof GM, Gomes BC. Livin, a novel inhibitor of apoptosis protein family member. J Biol Chem. 2001;276(5):3238-46. |
| Immunogen | Livin antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Livin. The immunogen is located within the last 50 amino acids of Livin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Livin antibody can be used for detection of Livin by Western blot at 0.5 μg/mL. A band at 33 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BIRC7 |
|---|---|
| Alternative Names | Livin Antibody: KIAP LIVIN MLIAP RNF50 ML-IAP KIAP UNQ5800/PRO19607/PRO21344 Baculoviral IAP repeat-containing protein 7 Kidney inhibitor of apoptosis protein |
| Molecular Weight(MW) | 33 kDa |
| Sequence Similarities | 3) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | A lower but much weaker band at 30 kDa was detected in Raji cell lysate, which may represent the short form of Livin. |
Application
Immunofluorescence of Livin in Human Small Intestine cells with Livin antibody at 20 μg/mL.
Application
Immunohistochemistry of Livin in human small intestine tissue with Livin antibody at 5 μg/mL.
Application
Western blot analysis of Livin expression in human Raji cell lysate with Livin antibody at 0.5 μg/mL.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | Livin antibody can be used for detection of Livin by Western blot at 0.5 μg/mL. A band at 33 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Livin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Livin Antibody is supplied in PBS containing 0.02% sodium azide. |
