Application
Immunohistochemistry of LMBRD1 in human brain tissue with LMBRD1 antibody at 2.5 μg/mL.Application
Immunofluorescence of LMBRD1 in human brain tissue with LMBRD1 antibody at 20 μg/mL.Application
Western blot analysis of LMBRD1 in human brain tissue lysate with LMBRD1 antibody at 1 μg/mL.| Product Name | LMBRD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LMBRD1 Antibody: NESI, LMBD1, MAHCF, C6orf209, NESI, BM-021, CD001, MSTP044, Probable lysosomal cobalamin transporter, HDAg-L-interacting protein NESI |
| Product description | LMBRD1 Antibody: LMBRD1, also known as NESI (nuclear export signal-interacting protein, is a lysosomal membrane protein that is thought be involved in the transport and metabolism of cobalamin. LMBRD1 was initially identified as interacting with the large form of the hepatitis delta antigen and may be required for the nucleocytoplasmic shuttling of the hepatitis delta virus. Mutations in this gene are associated with the vitamin B12 metabolism disorder termed, homocystinuria-megaloblastic anemia complementation type F (cblF).1) Gailus S, Hohne W, Gasnier B, et al. Insights into lysosomal cobalamin trafficking: lessons learned from cblF disease. J. Mol. Med.2010; 88:459-66. |
| Immunogen | LMBRD1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human LMBRD1. The immunogen is located within the last 50 amino acids of LMBRD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LMBRD1 antibody can be used for detection of LMBRD1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LMBRD1 |
|---|---|
| Alternative Names | LMBRD1 Antibody: NESI LMBD1 MAHCF C6orf209 NESI BM-021 CD001 MSTP044 Probable lysosomal cobalamin transporter HDAg-L-interacting protein NESI |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of LMBRD1 in human brain tissue with LMBRD1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of LMBRD1 in human brain tissue with LMBRD1 antibody at 20 μg/mL.
Application
Western blot analysis of LMBRD1 in human brain tissue lysate with LMBRD1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | LMBRD1 antibody can be used for detection of LMBRD1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LMBRD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LMBRD1 Antibody is supplied in PBS containing 0.02% sodium azide. |
