Application
Antibody is used in Western blot to detect LOK in Jurkat cell lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | LOK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase 10, Lymphocyte-oriented kinase, STK10, LOK |
| Product description | This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway.1) Kuramochi, S., et al., Immunogenetics 49(5):369-375 (1999). |
| Immunogen | This LOK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 692-721 amino acids from the C-terminal region of human LOK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STK10 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase 10 Lymphocyte-oriented kinase STK10 LOK |
| Molecular Weight(MW) | 112 kDa |
Application
Antibody is used in Western blot to detect LOK in Jurkat cell lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
