Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
LPPR4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in Hela,Jurkat,NCI-H460 cell line lysates (35ug/lane).| Product Name | LPPR4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lipid phosphate phosphatase-related protein type 4, Brain-specific phosphatidic acid phosphatase-like protein 1, Plasticity-related gene 1 protein, PRG-1, LPPR4, KIAA0455, PRG1 |
| Product description | LPPR4 belongs to the lipid phosphate phosphatase (LPP) family. LPPs catalyze the dephosphorylation of a number of bioactive lipid mediators that regulate a variety of cell functions. This protein is specifically expressed in neurons. It is located in the membranes of outgrowing axons and has been shown to be important for axonal outgrowth during development and regenerative sprouting.1) Trimbuch, T., et al. Cell 138(6):1222-1235(2009) |
| Immunogen | This LPPR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 666-695 amino acids from the C-terminal region of human LPPR4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LPPR4 |
|---|---|
| Alternative Names | Lipid phosphate phosphatase-related protein type 4 Brain-specific phosphatidic acid phosphatase-like protein 1 Plasticity-related gene 1 protein PRG-1 LPPR4 KIAA0455 PRG1 |
| Molecular Weight(MW) | 83 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
LPPR4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in Hela,Jurkat,NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
