Application
Immunofluorescence of LRFN1 in mouse brain tissue with LRFN1 antibody at 20 μg/mL.Application
Immunohistochemistry of LRFN1 in mouse brain tissue with LRFN1 antibody at 2.5 μg/mL.Application
Western blot analysis of LRFN1 in human brain lysate with LRFN1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | LRFN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LRFN1 Antibody: SALM2, KIAA1484, SALM2, Leucine-rich repeat and fibronectin type III domain-containing protein 1, Synaptic adhesion-like molecule 2 |
| Product description | LRFN1 Antibody: LRFN1 is one of a family of five transmembrane glycoproteins that are highly expressed in neuronal tissues. LRFN proteins share leucine-rich repeat (LRR)-immunoglobulin-like (Ig)-fibronectin type III (Fn)-transmembrane domain structure with other members of the LRR-Ig-Fn protein superfamily such as the Slitrk family of proteins. Expression of LRFN1, -3, and -4 mRNA was detected in embryonic neuronal cells, while Lrfn2 and Lrfn5 expression was primarily restricted to more mature cells. LRFN1, -2, and -4 bound to PDZ domains of postsynaptic PSD95, re-distributing PSD95 to the cell periphery. It has been suggested that the Lrfn proteins play a role in the developing and/or mature vertebrate nervous system. At least two isoforms of LRFN1 are known to exist.1) Morimura N, Inoue T, Katayama K, et al. Comparative analysis of structure, expression and PSD95-binding capacity of Lrfn, a novel family of neuronal transmembrane proteins. Gene2006; 380:72-83. |
| Immunogen | LRFN1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human LRFN1. The immunogen is located within amino acids 700 - 750 of LRFN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LRFN1 antibody can be used for detection of LRFN1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LRFN1 |
|---|---|
| Alternative Names | LRFN1 Antibody: SALM2 KIAA1484 SALM2 Leucine-rich repeat and fibronectin type III domain-containing protein 1 Synaptic adhesion-like molecule 2 |
| Tissue Specificity | This antibody is predicted to not cross-react with other members of the LRFN family. |
Application
Immunofluorescence of LRFN1 in mouse brain tissue with LRFN1 antibody at 20 μg/mL.
Application
Immunohistochemistry of LRFN1 in mouse brain tissue with LRFN1 antibody at 2.5 μg/mL.
Application
Western blot analysis of LRFN1 in human brain lysate with LRFN1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | LRFN1 antibody can be used for detection of LRFN1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LRFN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LRFN1 Antibody is supplied in PBS containing 0.02% sodium azide. |
