Application
Immunohistochemistry of LRFN5 in mouse brain tissue with LRFN5 antibody at 5 μg/mL.Application
Western blot analysis of LRFN5 in EL4 cell lysate with LRFN5 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | LRFN5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | LRFN5 Antibody: LRFN5 is one of a family of five transmembrane glycoproteins that are highly expressed in neuronal tissues. LRFN proteins share leucine-rich repeat (LRR)-immunoglobulin-like (Ig)-fibronectin type III (Fn)-transmembrane domain structure with other members of the LRR-Ig-Fn protein superfamily such as the Slitrk family of proteins. Expression of LRFN1, -3, and -4 mRNA was detected in embryonic neuronal cells, while LRFN2 and LRFN5 expression was primarily restricted to more mature cells. LRFN1, -2, and -4 bound to PDZ domains of postsynaptic PSD95, re-distributing PSD95 to the cell periphery. It has been suggested that the Lrfn proteins play a role in the developing and/or mature vertebrate nervous system.1) Morimura N, Inoue T, Katayama K, et al. Comparative analysis of structure, expression and PSD95-binding capacity of Lrfn, a novel family of neuronal transmembrane proteins. Gene2006; 380:72-83. |
| Immunogen | LRFN5 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human LRFN5. The immunogen is located within amino acids 650 - 700 of LRFN5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,IHC-P,WB |
| LRFN5 antibody can be used for detection of LRFN5 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LRFN5 |
|---|---|
| Alternative Names | LRFN5 Antibody: SALM5 FIGLER8 C14orf146 SALM5 Leucine-rich repeat and fibronectin type-III domain-containing protein 5 |
| Tissue Specificity | At least two isoforms of LRFN5 are known to exist. This antibody is predicted to not cross-react with other members of the LRFN family. |
Application
Immunohistochemistry of LRFN5 in mouse brain tissue with LRFN5 antibody at 5 μg/mL.
Application
Western blot analysis of LRFN5 in EL4 cell lysate with LRFN5 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | LRFN5 antibody can be used for detection of LRFN5 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | LRFN5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LRFN5 Antibody is supplied in PBS containing 0.02% sodium azide. |
