Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of LRP12 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of lysate from SW480 cell line,using LRP12 Antibody at 1:1000 at each laneApplication
LRP12 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | LRP12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Low-density lipoprotein receptor-related protein 12, LRP-12, Suppressor of tumorigenicity 7 protein, LRP12, ST7 |
| Product description | This gene encodes a member of the low-density lipoprotein receptor related protein family. The product of this gene is a transmembrane protein that is differentially expressed in many cancer cells. Alternate splicing results in multiple transcript variants.1) Garnis, C., et al. Oncogene 23(14):2582-2586(2004) |
| Immunogen | This LRP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 635-662 amino acids from the C-terminal region of human LRP12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LRP12 |
|---|---|
| Alternative Names | Low-density lipoprotein receptor-related protein 12 LRP-12 Suppressor of tumorigenicity 7 protein LRP12 ST7 |
| Molecular Weight(MW) | 95 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of LRP12 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of lysate from SW480 cell line,using LRP12 Antibody at 1:1000 at each lane
Application
LRP12 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
