Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
LRRC45 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Immunofluorescent analysis of U251 cells, using LRRC45 Antibody . Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).Application
Western blot analysis of lysates from human blood plasma tissue (from left to right),using LRRC45 Antibody .AP11368b was diluted at 1:1000 at each lane.| Product Name | LRRC45 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Leucine-rich repeat-containing protein 45, LRRC45 |
| Product description | 1) Olsen, J.V., et al. Cell 127(3):635-648(2006) Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) : |
| Immunogen | This LRRC45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 615-643 amino acids from the C-terminal region of human LRRC45. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:25 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LRRC45 |
|---|---|
| Alternative Names | Leucine-rich repeat-containing protein 45 LRRC45 |
| Molecular Weight(MW) | 76 kDa |
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
LRRC45 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Immunofluorescent analysis of U251 cells, using LRRC45 Antibody . Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Application
Western blot analysis of lysates from human blood plasma tissue (from left to right),using LRRC45 Antibody .AP11368b was diluted at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:25 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
