Application
Immunohistochemistry of LRRC8A in rat spleen tissue with LRRC8A antibody at 2.5 μg/mL.Application
Western blot analysis of LRRC8A in EL4 cell lysate with LRRC8A antibody at (A) 1 and (B) 2 μg/ml.| Product Name | LRRC8A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Leucine rich repeat containing 8 family member A, leucine-rich repeat-containing protein 8A, LRRC8, AGM5 |
| Product description | The leucine rich repeat containing 8 family member A protein (LRRC8A) belongs the LRRC8 family of proteins within the LRR superfamily of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions (1,2). LRRC8A is a four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation (1). LRRC8A has also been shown to be an essential component of the volume-regulated anion channel VRAC (3).1) Sawada A, Takihara Y, Kim JY, et al. A congenital mutation of the novel gene LRRC8 causes agammaglobulinemia in humans. J. Clin. Invest. 2003; 112:1707-13. |
| Immunogen | LRRC8A antibody was raised against a 15 amino acid peptide near the amino terminus of human LRRC8A. The immunogen is located within amino acids 60 - 110 of LRRC8A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| LRRC8A antibody can be used for detection of LRRC8A by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LRRC8A |
|---|---|
| Alternative Names | Leucine rich repeat containing 8 family member A leucine-rich repeat-containing protein 8A LRRC8 AGM5 |
| Molecular Weight(MW) | Predicted: 89 kDa Observed: 95 kDa |
| Tissue Specificity | LRRC8A antibody is human, mouse and rat reactive. |
Application
Immunohistochemistry of LRRC8A in rat spleen tissue with LRRC8A antibody at 2.5 μg/mL.
Application
Western blot analysis of LRRC8A in EL4 cell lysate with LRRC8A antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | LRRC8A antibody can be used for detection of LRRC8A by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LRRC8A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | LRRC8A antibody is supplied in PBS containing 0.02% sodium azide. |
