Application
Immunohistochemistry of LRRTM4 in human brain tissue with LRRTM4 antibody at 2.5 μg/mL.Application
Western blot analysis of LRRTM4 in HeLa cell lysate with LRRTM4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of LRRTM4 in human brain tissue with LRRTM4 antibody at 20 μg/mL.| Product Name | LRRTM4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LRRTM4 Antibody: Leucine-rich repeat transmembrane neuronal protein 4 |
| Product description | LRRTM4 Antibody: The Leucine-rich repeat transmembrane neuronal proteins (LRRTMs) are differentially expressed in the nervous system and were recently found to instruct presynaptic and mediate postsynaptic glutamatergic differentiation, with LRRTM1 and LRRTM2 most potent at inducing presynaptic differentiation. Little is known about the function of LRRTM4; in mouse, it is expressed in the limb mesenchyme, neural tube, caudal mesoderm and in three distinct regions of the head. Later expression occurs in a subset of the developing sclerotome. Its similarity to other LRRTM family members suggests that LRRTM4 may also play a role in the development and maintenance of the vertebrate nervous system.1) Lauren J, Airaksinen MS, Saarma M, et al. A novel gene family encoding leucine-rich repeat transmembrane protein differentially expressed in the nervous system. Genomics 2003; 81:411-21. |
| Immunogen | LRRTM4 antibody was raised against a 16 amino acid synthetic peptide near the center of human LRRTM4. The immunogen is located within amino acids 350 - 400 of LRRTM4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| LRRTM4 antibody can be used for detection of LRRTM4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LRRTM4 |
|---|---|
| Alternative Names | LRRTM4 Antibody: Leucine-rich repeat transmembrane neuronal protein 4 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least two isoforms of LRRTM4 are known to exist; this antibody will both isoforms. LRRTM4 antibody is predicted to not cross-react with other LRRTM family members. |
| Entrez Gene | 80059 |
|---|---|
| Protein Accession | NP_001128217 |
Application
Immunohistochemistry of LRRTM4 in human brain tissue with LRRTM4 antibody at 2.5 μg/mL.
Application
Western blot analysis of LRRTM4 in HeLa cell lysate with LRRTM4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of LRRTM4 in human brain tissue with LRRTM4 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | LRRTM4 antibody can be used for detection of LRRTM4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LRRTM4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LRRTM4 Antibody is supplied in PBS containing 0.02% sodium azide. |
