Application
Immunohistochemical analysis of paraffin - embedded human lung carcinoma (top) and kidney carcinoma (bottom) showing nuclear localization using LSD1 antibody with DAB staining.Application
Western blot analysis using LSD1 monoclonal antibody against truncated LSD1 recombinant protein Hela cell lysate. and Jurkat cell lysate .| Product Name | LSD1AOF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AOF2, KDM1, KIAA0601, LSD1, BRAF35-HDAC complex protein BHC110AOF2, BHC110 |
| Product description | The amine oxidase domain 2（AOF2）gene encodes a nuclear protein(LSD1,~95 kDa) containing a Swirm domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. LSD1 is a chromatin-modifying enzyme, which serve as a docking module for the stabilization of the associated corepressor complex(es) on chromatin.1) Shi YJ, et.al Mol Cell. 2005 Sep 16;19(6):857-64. |
| Immunogen | Ni-NTA purified truncated recombinant human LSD1 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | KDM1A |
|---|---|
| Alternative Names | AOF2 KDM1 KIAA0601 LSD1 BRAF35-HDAC complex protein BHC110AOF2 BHC110 |
| Sequence Similarities | 2) Cat. No. 1205 - Jurkat Cell Lysate |
Application
Immunohistochemical analysis of paraffin - embedded human lung carcinoma (top) and kidney carcinoma (bottom) showing nuclear localization using LSD1 antibody with DAB staining.
Application
Western blot analysis using LSD1 monoclonal antibody against truncated LSD1 recombinant protein Hela cell lysate. and Jurkat cell lysate .| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LSD1/AOF2 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
