Application
Immunofluorescence of LXR-A in rat liver tissue with LXR-A antibody at 20 μg/mL.Application
Immunohistochemistry of LXR-A in rat liver tissue with LXR-A antibody at 5 μg/mL.Application
Western blot analysis of LXR-A in rat liver tissue lysate with LXR-A antibody at (A) 1 and (B) 2 μg/mL.| Product Name | LXR-A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LXR-A Antibody: LXRA, LXR-a, RLD-1, LXRA, Oxysterols receptor LXR-alpha, Liver X receptor alpha |
| Product description | LXR-A Antibody: LXR-A belongs to the Liver X Receptor family that encodes highly homologous transcription factors. Like the highly homologous LXR-B, LXR-A forms heterodimers with the retinoic acid receptor RXRalpha, function as sensors for cellular oxysterols which when activated, increase the expression of genes that control sterol and fatty acid metabolism and homeostasis. Recent experiments have indicated that the LXRs can also modulate both innate and adaptive immune responses. Human and mouse tumors produce LXR ligands that inhibit CCR7 expression on maturing dendritic cells (DCs), thereby allowing tumor immunoescape. In mouse models, it was shown that ablating LXR-A signaling led to an immune-mediated strong inhibition of tumor growth, suggesting that manipulation of this pathway may be a viable anti-cancer approach.1) Willy PJ, Umesono K, Ong ES, et al. LXR, a nuclear receptor that defines a distinct retinoid response pathway. Genes Dev.1995; 9:1033-45. |
| Immunogen | LXR-A antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus human LXR-A. The immunogen is located within amino acids 50 - 100 of LXR-A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| LXR-A antibody can be used for detection of LXR-A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR1H3 |
|---|---|
| Alternative Names | LXR-A Antibody: LXRA LXR-a RLD-1 LXRA Oxysterols receptor LXR-alpha Liver X receptor alpha |
| Molecular Weight(MW) | Predicted: 44, 49 kDa Observed: 46 kDa |
Application
Immunofluorescence of LXR-A in rat liver tissue with LXR-A antibody at 20 μg/mL.
Application
Immunohistochemistry of LXR-A in rat liver tissue with LXR-A antibody at 5 μg/mL.
Application
Western blot analysis of LXR-A in rat liver tissue lysate with LXR-A antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | LXR-A antibody can be used for detection of LXR-A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | LXR-A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | LXR-A Antibody is supplied in PBS containing 0.02% sodium azide. |
