Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with CDH15 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of CDH15 using rabbit polyclonal CDH15 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH15 gene (Lane 2).Application
Western Blot at 1:8000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | M Cadherin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cadherin-15, Cadherin-14, Muscle cadherin, M-cadherin, CDH15, CDH14, CDH3 |
| Product description | CDH15 is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. This protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation.1) Kang,J.S., Proc. Natl. Acad. Sci. U.S.A. 100 (7), 3989-3994 (2003) Hollnagel,A., Mol. Cell. Biol. 22 (13), 4760-4770 (2002) Meigs,T.E., Proc. Natl. Acad. Sci. U.S.A. 98 (2), 519-524 (2001) |
| Immunogen | This M Cadherin (CDH15) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 739-767 amino acids from the C-terminal region of human M Cadherin (CDH15). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDH15 |
|---|---|
| Alternative Names | Cadherin-15 Cadherin-14 Muscle cadherin M-cadherin CDH15 CDH14 CDH3 |
| Molecular Weight(MW) | 89 kDa |
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with CDH15 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of CDH15 using rabbit polyclonal CDH15 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH15 gene (Lane 2).
Application
Western Blot at 1:8000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
