Application
Sirt3 Antibody IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of Sirt3 Antibody in mouse brain tissue lysates (35ug/lane)Application
Western blot analysis of Sirt3 Antibody in CHO cell line lysates (35ug/lane)Application
Western blot analysis of lysate from mouse heart tissue lysate, using Mouse Sirt3 Antibody at 1:1000.Application
Western Blot at 1:2000 dilution + CHO whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:64000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.| Product Name | M Sirt3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NAD-dependent protein deacetylase sirtuin-3, 351-, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, mSIR2L3, Sirt3, Sir2l3 |
| Product description | SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.1) Hirschey, M.D., et al. Nature 464(7285):121-125(2010) |
| Immunogen | This Sirt3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of Mouse Sirt3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HamsterMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Sirt3 |
|---|---|
| Alternative Names | NAD-dependent protein deacetylase sirtuin-3 351- Regulatory protein SIR2 homolog 3 SIR2-like protein 3 mSIR2L3 Sirt3 Sir2l3 |
| Molecular Weight(MW) | 37 kDa |
Application
Sirt3 Antibody IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of Sirt3 Antibody in mouse brain tissue lysates (35ug/lane)
Application
Western blot analysis of Sirt3 Antibody in CHO cell line lysates (35ug/lane)
Application
Western blot analysis of lysate from mouse heart tissue lysate, using Mouse Sirt3 Antibody at 1:1000.
Application
Western Blot at 1:2000 dilution + CHO whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:64000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
