Application
Immunofluorescence of MACC1 in Human Liver cells with MACC1 antibody at 20 μg/mL.Application
Immunohistochemistry of MACC1 in human liver tissue with MACC1 antibody at 2.5 μg/mL.Application
Western blot analysis of MACC1 in mouse liver tissue lysate with MACC1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | MACC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MACC1 Antibody: Metastasis associated in colon cancer 1 (MACC1) is a key regulator of the hepatocyte growth factor (HGF)-HGF receptor (MET) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. MACC1 protein consists of four domains: ZU5, SH3, and two C-terminal death domains (DD). Expression of MACC1 was found significantly upregulated in malignant tissues (colon cancer of all stages as well as liver and lung metastases) compared to normal tissues or adenomas. MACC1 represents an early and crucial prognostic indicator for colon cancer metastasis that is independent of age, sex, tumor infiltration, nodal status, and lymph vessel invasion. Besides its involvement in signal transduction with the MET receptor, MACC1 also links MET signaling and apoptosis. MACC1 may also be an important therapeutic target for colorectal cancer treatment.1) Stein U, Walther W, Arlt F, et al. MACC1, a newly identified key regulator of HGF-MET signaling, predicts colon cancer metastasis. Nat. Med.2009; 15:59-67. |
| Immunogen | MACC1 antibody was raised against a 17 amino acid synthetic peptide near the center of human MACC1. The immunogen is located within amino acids 430 - 480 of MACC1. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MACC1 antibody can be used for detection of MACC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MACC1 |
|---|---|
| Alternative Names | MACC1 Antibody: 7A5 SH3BP4L Metastasis-associated in colon cancer protein 1 SH3 domain-containing protein 7a5 |
| Molecular Weight(MW) | Predicted: 94 kDa Observed: 90 kDa |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
| Tissue Specificity | At least two isoforms of MACC1 are known to exist. |
Application
Immunofluorescence of MACC1 in Human Liver cells with MACC1 antibody at 20 μg/mL.
Application
Immunohistochemistry of MACC1 in human liver tissue with MACC1 antibody at 2.5 μg/mL.
Application
Western blot analysis of MACC1 in mouse liver tissue lysate with MACC1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1404 - Mouse Liver Tissue Lysate |
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| Application Notes | MACC1 antibody can be used for detection of MACC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MACC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MACC1 Antibody is supplied in PBS containing 0.02% sodium azide. |
