Application
Immunofluorescence of Maelstrom in HeLa cells with Maelstrom antibody at 20 μg/mL.Application
Immunocytochemistry of Maelstrom in HeLa cells with Maelstrom antibody at 5 μg/mL.Application
Western blot analysis of Maelstrom in HeLa cell lysate with Maelstrom antibody at 1 μg/mL.| Product Name | Maelstrom Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Maelstrom Antibody: CT128, SPATA35, Protein maelstrom homolog |
| Product description | Maelstrom Antibody: The mammalian homolog of the Drosophila protein Maelstrom is expressed in the male germline and localizes to the sex body in spermatocytes and the chromatoid body in round spermatids. Similar to its expression in Drosophila, Maelstrom is a component of nuages, a germ-cell specific organelle and is thought to be essential for spermatogenesis and transposon repression during meiosis. In humans, Maelstrom has been found to be expressed only in the testis and in various cancer cell lines. Treatment of these cell lines with the demethylating agent 5'-Aza-2-Deoxycytidine significantly upregulated Maelstrom levels, indicating that its expression is regulated by DNA methylation.1) Clegg NJ, Frost DM, Larkin MK, et al. Maelstrom is required for an early step in the establishment of Drosophila oocyte polarity: posterior localization of grk mRNA. Development1997; 124:4661-71. |
| Immunogen | Maelstrom antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human Maelstrom. The immunogen is located within amino acids 30 - 80 of Maelstrom. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Maelstrom antibody can be used for detection of Maelstrom by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAEL |
|---|---|
| Alternative Names | Maelstrom Antibody: CT128 SPATA35 Protein maelstrom homolog |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunofluorescence of Maelstrom in HeLa cells with Maelstrom antibody at 20 μg/mL.
Application
Immunocytochemistry of Maelstrom in HeLa cells with Maelstrom antibody at 5 μg/mL.
Application
Western blot analysis of Maelstrom in HeLa cell lysate with Maelstrom antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | Maelstrom antibody can be used for detection of Maelstrom by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Maelstrom antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Maelstrom Antibody is supplied in PBS containing 0.02% sodium azide. |
