Application
Antibody used in WB on Human HepG2 at 5.0 ug/ml.| Product Name | MAFF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAFF, U-MAF, hMafF |
| Product description | MAFF is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. MAFF can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. MAFF may also be involved in the cellular stress response.The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Two transcript variants encoding the same protein have been found for this gene.1) Marini, M.G., (2002) Blood Cells Mol. Dis. 29 (2), 145-158. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MAFF. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MAFF antibody can be used for detection of MAFF by ELISA at 1:62500. MAFF antibody can be used for detection of MAFF by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogDrosophilaHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAFF |
|---|---|
| Alternative Names | MAFF U-MAF hMafF |
| Molecular Weight(MW) | 18 kDa, 18 kDa, 18 kDa, 18 kDa, 18 kDa |
Application
Antibody used in WB on Human HepG2 at 5.0 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | MAFF antibody can be used for detection of MAFF by ELISA at 1:62500. MAFF antibody can be used for detection of MAFF by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MAFF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
