Application
Western blot analysis of anti-MAGEA11 Antibody pre-incubated with and without blocking peptide in 293 cell line lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | MAGEA11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Melanoma-associated antigen 11, Cancer/testis antigen 111, CT111, MAGE-11 antigen, MAGEA11, MAGE11 |
| Product description | MAGEA11 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.1) Rogner, U.C., et al., Genomics 29(3):725-731 (1995). |
| Immunogen | This MAGEA11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-228 amino acids from the N-terminal region of human MAGEA11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | MAGEA11 |
|---|---|
| Alternative Names | Melanoma-associated antigen 11 Cancer/testis antigen 111 CT111 MAGE-11 antigen MAGEA11 MAGE11 |
| Molecular Weight(MW) | 48 kDa |
Application
Western blot analysis of anti-MAGEA11 Antibody pre-incubated with and without blocking peptide in 293 cell line lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
