Application
Western blot analysis in K562 cell line lysates (35ug/lane).Application
MAGEA12 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | MAGEA12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Melanoma-associated antigen 12, Cancer/testis antigen 112, CT112, MAGE-12 antigen, MAGE12F antigen, MAGEA12, MAGE12 |
| Product description | MAGEA12 is a member of the melanoma antigen gene (MAGE) family. The proteins of this family are tumor-specific antigens that can be recognized by autologous cytolytic T lymphocytes. This gene is expressed in various tumors and tumor cell lines from different tissue origins, but not detected in normal tissues, except testis. The function of this gene is unknown. This and other MAGE genes form a gene cluster at chromosome Xq28 region.1) Mallon, A.M., et al., Genome Res. 10(6):758-775 (2000). |
| Immunogen | This MAGEA12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the N-terminal region of human MAGEA12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAGEA12 |
|---|---|
| Alternative Names | Melanoma-associated antigen 12 Cancer/testis antigen 112 CT112 MAGE-12 antigen MAGE12F antigen MAGEA12 MAGE12 |
| Molecular Weight(MW) | 35 kDa |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).
Application
MAGEA12 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
