Application
Antibody is used in Western blot to detect MAGEA3 in Jurkat lysate.Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
MAGEA3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | MAGEA3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Melanoma-associated antigen 3, Antigen MZ2-D, Cancer/testis antigen 13, CT13, MAGE-3 antigen, MAGEA3, MAGE3 |
| Product description | MAGEA3 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.1) Guo, J., et al., Exp. Mol. Pathol. 74(2):140-147 (2003). Consogno, G., et al., Blood 101(3):1038-1044 (2003). Rogner, U.C., et al., Genomics 29(3):725-731 (1995). Gaugler, B., et al., J. Exp. Med. 179(3):921-930 (1994). van der Bruggen, P., et al., Science 254(5038):1643-1647 (1991). |
| Immunogen | This MAGEA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-314 amino acids from the C-terminal region of human MAGEA3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | MAGEA3 |
|---|---|
| Alternative Names | Melanoma-associated antigen 3 Antigen MZ2-D Cancer/testis antigen 13 CT13 MAGE-3 antigen MAGEA3 MAGE3 |
| Molecular Weight(MW) | 35 kDa |
Application
Antibody is used in Western blot to detect MAGEA3 in Jurkat lysate.
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
MAGEA3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
