Application
Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 μg/ml.Application
Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 μg/ml.Application
Immunocytochemistry of MAGEA4 in HeLa cells with MAGEA4 antibody at 5 μg/ml.Application
Immunofluorescence of MAGEA4 inHeLa cells with MAGEA4 antibody at 20 μg/ml.Application
Immunohistochemistry of MAGEA4 in human breast tumor with MAGEA4 antibody at 5 μg/mL.Application
Western blot analysis of MAGEA4 in A431 cell lysate with MAGEA4 antibody at 1 μg/ml.| Product Name | MAGEA4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Melanoma antigen family A4, MAGE4, MAGE-41, MAGE4A, MAGE-4 antigen, MAGE4B, MAGE-X2, MAGE-X2 antigen, Melanoma-associated antigen 4 |
| Product description | MAGEA4 is a member of the melanoma-associated antigen family that consists of a number of antigens recognized by cytotoxic T lymphocytes (1). MAGEA4 may serve as a target for antitumoral vaccination and may play a role in embryonal development and tumor transformation or aspects of tumor progression. It is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma and have been implicated in some hereditary disorders, such as dyskeratosis congenital (2-4).1) Bhan S, Negi SS, Shao C, et al. BORIS binding to the promoters of cancer testis antigens, MAGEA2, MAGEA3, and MAGEA4, is associated with their transcriptional activation in lung cancer. Clin. Cancer Res. 2011; 17:4267-76. |
| Immunogen | MAGEA4 antibody was raised against a 16 amino acid peptide near the amino terminus of human MAGEA4. The immunogen is located within the first 50 amino acids of MAGEA4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MAGEA4 antibody can be used for detection of MAGEA4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAGEA4 |
|---|---|
| Alternative Names | Melanoma antigen family A4 MAGE4 MAGE-41 MAGE4A MAGE-4 antigen MAGE4B MAGE-X2 MAGE-X2 antigen Melanoma-associated antigen 4 |
| Molecular Weight(MW) | Predicted: 35 kDa Observed: 45 kDa |
| Sequence Similarities | 2) Cat. No. 10-001 - Human Breast Tissue Slide |
| Tissue Specificity | MAGEA4 antibody is human specific. At least four isoforms of MAGEA4 are known to exist. |
| Entrez Gene | 4103 |
|---|---|
| Protein Accession | NP_001011550 |
Application
Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 μg/ml.
Application
Immunofluorescence of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 μg/ml.
Application
Immunocytochemistry of MAGEA4 in HeLa cells with MAGEA4 antibody at 5 μg/ml.
Application
Immunofluorescence of MAGEA4 inHeLa cells with MAGEA4 antibody at 20 μg/ml.
Application
Immunohistochemistry of MAGEA4 in human breast tumor with MAGEA4 antibody at 5 μg/mL.
Application
Western blot analysis of MAGEA4 in A431 cell lysate with MAGEA4 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | MAGEA4 antibody can be used for detection of MAGEA4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | MAGEA4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | MAGEA4 antibody is supplied in PBS containing 0.02% sodium azide. |
