Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Antibody is used in Western blot to detect MAGED1 in A549 lysate.| Product Name | MAGED1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Melanoma-associated antigen D1, MAGE tumor antigen CCF, MAGE-D1 antigen, Neurotrophin receptor-interacting MAGE homolog, MAGED1, NRAGE |
| Product description | MAGED1 is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway.1) Sasaki, A., et al., J. Biol. Chem. 277(25):22541-22546 (2002). |
| Immunogen | This MAGED1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426-456 amino acids from the Central region of human MAGED1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | MAGED1 |
|---|---|
| Alternative Names | Melanoma-associated antigen D1 MAGE tumor antigen CCF MAGE-D1 antigen Neurotrophin receptor-interacting MAGE homolog MAGED1 NRAGE |
| Molecular Weight(MW) | 86 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Antibody is used in Western blot to detect MAGED1 in A549 lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
