Application
Western blot analysis of MAGEH1 using rabbit polyclonal MAGEH1 Antibody (D201) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAGEH1 gene.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Antibody is used in Western blot to detect MAGEH1 in Jurkat cell lysate.| Product Name | MAGEH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Melanoma-associated antigen H1, Apoptosis-related protein 1, APR-1, MAGE-H1 antigen, Restin, MAGEH1, APR1 |
| Product description | Melanoma-associated antigen (MAGE) are completely silent in normal tissues, with the exception of male germ cells, and, for some of them, placenta. These antigens ought to be strictly tumor specific, expressed in tumor cells of various histological types. Because of their specific expression on tumor cells, these antigens are of particular interest for antitumor immunotherapy. Genes of the MAGE family direct the expression of tumor antigens that are recognized on a human melanoma by autologous cytolytic T lymphocytes. Though the function of MAGEH1 is not fully elucidated, it may play a role in embryonal development and tumor transformation or aspects of tumor progression.1) Chomez, P., et al., Cancer Res. 61(14):5544-5551 (2001). |
| Immunogen | This MAGEH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-218 amino acids from the C-terminal region of human MAGEH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | MAGEH1 |
|---|---|
| Alternative Names | Melanoma-associated antigen H1 Apoptosis-related protein 1 APR-1 MAGE-H1 antigen Restin MAGEH1 APR1 |
| Molecular Weight(MW) | 24 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of MAGEH1 using rabbit polyclonal MAGEH1 Antibody (D201) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAGEH1 gene.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Antibody is used in Western blot to detect MAGEH1 in Jurkat cell lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
