Application
Western blot analysis in 293 cell line lysates (35ug/lane).Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MAN2A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a protein which is a member of family 38 of the glycosyl hydrolases. The protein is located in the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus.1) Han, S., et al. Leuk. Res. 34(10):1271-1274(2010) |
| Immunogen | This MAN2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 456-482 amino acids from the Central region of human MAN2A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAN2A1 |
|---|---|
| Alternative Names | Alpha-mannosidase 2 Golgi alpha-mannosidase II AMan II Man II Mannosidase alpha class 2A member 1 Mannosyl-oligosaccharide 1 3-1 6-alpha-mannosidase MAN2A1 MANA2 |
| Molecular Weight(MW) | 131 kDa |
Application
Western blot analysis in 293 cell line lysates (35ug/lane).
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
