Application
Immunofluorescence of MANF in Human Brain tissue with MANF antibody at 20 μg/mL.Application
Immunohistochemistry of MANF in human brain tissue with MANF antibody at 2.5 μg/mL.Application
Western blot analysis of MANF in rat brain tissue lysate with MANF antibody at (A) 1 and (B) 2 μg/mL.| Product Name | MANF Antibody |
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| Antibody Type | Primary Antibodies |
| Product description | MANF Antibody: MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson's disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.1) Shridhar V, Rivard S, Shridhar R, et al. A gene from human chromosomal band 3p21.1 encodes a highly conserved arginine-rich protein and is mutated in renal cell carcinomas. Oncogene 1996; 12:1931-9. |
| Immunogen | MANF antibody was raised against a 12 amino acid synthetic peptide from near the amino terminus of human MANF. The immunogen is located within the first 50 amino acids of MANF. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MANF antibody can be used for detection of MANF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARMET |
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| Alternative Names | MANF Antibody: ARP ARMET ARP Mesencephalic astrocyte-derived neurotrophic factor Arginine-rich protein |
| Molecular Weight(MW) | Predicted: 20 kDa Observed: 27 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | This antibody does not cross-react with CDNF. |
Application
Immunofluorescence of MANF in Human Brain tissue with MANF antibody at 20 μg/mL.
Application
Immunohistochemistry of MANF in human brain tissue with MANF antibody at 2.5 μg/mL.
Application
Western blot analysis of MANF in rat brain tissue lysate with MANF antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
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| Application Notes | MANF antibody can be used for detection of MANF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
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| Storage Instructions | MANF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MANF Antibody is supplied in PBS containing 0.02% sodium azide. |
