Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HCT15 cell lysate| Product Name | Rabbit anti-MANF polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARMET, ARP, MGC142148, MGC142150 |
| Immunogen | The immunogen for anti-MANF antibody: synthetic peptide directed towards the C terminal of human MANF |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MANF |
|---|---|
| Gene Synonyms | ARMET ARP MGC142148 MGC142150 |
| Gene Full Name | Mesencephalic astrocyte-derived neurotrophic factor |
| Gene Summary | MANF is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of MANF gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of polymorphisms in the arginine-rich region, including a specific mutation that changes the previously numbered codon 50 from ATG to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus not tumor-related. |
| Alternative Names | ARMET ARP MGC142148 MGC142150 |
| Molecular Weight(MW) | 21kDa |
| Sequence | 185 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HCT15 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
