WB
Western blot analysis of various lysates using ARMET/ARP/MANF Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.WB
Western blot analysis of various lysates using ARMET/ARP/MANF Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.ICC/IF
Immunofluorescence analysis of HeLa cellsusing ARMET/ARP/MANF Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of NIH/3T3cells using ARMET/ARP/MANF Rabbit pAb at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of PC-12 cellsusing ARMET/ARP/MANF Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of U2OS cellsusing ARMET/ARP/MANF Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human tonsil usingARMET/ARP/MANF Rabbit pAb at dilution of 1:100 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse spleen usingARMET/ARP/MANF Rabbit pAb at dilution of 1:100 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat spleen usingARMET/ARP/MANF Rabbit pAb at dilution of 1:100 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.IP
Immunoprecipitation analysis of 300 μg extracts of HepG2 cells using 3 μg ARMET/ARP/MANF antibody. Western blot was performed from the immunoprecipitate using ARMET/ARP/MANF antibody at a dilution of 1:1000.| Product Name | ARMET/ARP/MANF Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MANF |
|---|---|
| Gene Synonyms | ARP DDDS ARMET |
| Gene Full Name | mesencephalic astrocyte derived neurotrophic factor |
| Gene Summary | The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014] |
| Alternative Names | ARP ARMET ARMET/ARP/MANF |
| Molecular Weight(MW) | 21kDa(Observed band size:16kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of various lysates using ARMET/ARP/MANF Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
WB
Western blot analysis of various lysates using ARMET/ARP/MANF Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
ICC/IF
Immunofluorescence analysis of HeLa cellsusing ARMET/ARP/MANF Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of NIH/3T3cells using ARMET/ARP/MANF Rabbit pAb at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of PC-12 cellsusing ARMET/ARP/MANF Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of U2OS cellsusing ARMET/ARP/MANF Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human tonsil usingARMET/ARP/MANF Rabbit pAb at dilution of 1:100 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse spleen usingARMET/ARP/MANF Rabbit pAb at dilution of 1:100 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat spleen usingARMET/ARP/MANF Rabbit pAb at dilution of 1:100 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.
IP
Immunoprecipitation analysis of 300 μg extracts of HepG2 cells using 3 μg ARMET/ARP/MANF antibody. Western blot was performed from the immunoprecipitate using ARMET/ARP/MANF antibody at a dilution of 1:1000.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
