Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).Application
MAP3K15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:1000 dilution + human fetal brain lysate Lysates/proteins at 20 ug per lane.| Product Name | MAP3K15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mitogen-activated protein kinase kinase kinase 15, Apoptosis signal-regulating kinase 3, MAPK/ERK kinase kinase 15, MEK kinase 15, MEKK 15, MAP3K15, ASK3 |
| Product description | The protein encoded by this gene is a member of the mitogen-activated protein kinase (MAPK) family. These family members function in a protein kinase signal transduction cascade, where an activated MAPK kinase kinase (MAP3K) phosphorylates and activates a specific MAPK kinase (MAP2K), which then activates a specific MAPK. This MAP3K protein plays an essential role in apoptotic cell death triggered by cellular stresses. [provided by RefSeq].1) Kaji, T., et al. Biochem. Biophys. Res. Commun. 395(2):213-218(2010) |
| Immunogen | This MAP3K15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-342 amino acids from the N-terminal region of human MAP3K15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP3K15 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase kinase kinase 15 Apoptosis signal-regulating kinase 3 MAPK/ERK kinase kinase 15 MEK kinase 15 MEKK 15 MAP3K15 ASK3 |
| Molecular Weight(MW) | 147 kDa |
Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Application
MAP3K15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:1000 dilution + human fetal brain lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
