Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in Hela cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAPK1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | MAPK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MAPK1 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.1) Munshi, H.G., et al., J. Biol. Chem. 279(37):39042-39050 (2004). |
| Immunogen | This MAPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-345 amino acids from the C-terminal region of human MAPK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK1 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase 1 MAP kinase 1 MAPK 1 ERT1 Extracellular signal-regulated kinase 2 ERK-2 MAP kinase isoform p42 p42-MAPK Mitogen-activated protein kinase 2 MAP kinase 2 MAPK 2 MAPK1 ERK2 PRKM1 PRKM2 |
| Molecular Weight(MW) | 41 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in Hela cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAPK1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
